Search results for serum assay - 32 results

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High-throughput calcium flux assays: luminescent versus fluorescent readout

20 September 2016 | By Christophe Thierry (Galapagos NV), Jan Van der Schueren (Galapagos NV), Jans Mia (Galapagos NV), Nicolas Houvenaghel (Galapagos NV)

In vitro high-throughput cellular target-based assays are commonly used for hit identification targeting G-protein coupled receptors (GPCR). Agonist and antagonist identification of GPCRs often relies on the screening of large collections of chemicals using miniaturised in vitro cell-based functional assays. In the panel of assays, calcium mobilisation measured by aequorin…

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A high-throughput approach for measuring intracellular ATP levels to access compound-mediated cellular toxicity

30 November 2018 | By Maria Kuzikov (Fraunhofer-IME SP), Oliver Keminer (Fraunhofer Institute for Molecular Biology and Applied Ecology), Salma Hachim (Fraunhofer Institute for Molecular Biology and Applied Ecology), Sheraz Gul (Fraunhofer-IME SP)

Many drugs fail in clinical trials due to toxicity-related issues; therefore, drug-mediated cellular toxicity should be determined at an early stage in the drug discovery value chain. There are many assay systems that can be utilised to measure cellular toxicity. In this article we demonstrate that intracellular ATP concentrations are…

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High-throughput screening platforms incorporating physiologically relevant 3-D models

8 January 2017 | By Anton Simeonov, Madhu Lal-Nag

Two-dimensional monolayer cell proliferation assays for cancer drug discovery have enabled large-scale screens. But they represent a simplified view of oncogenes or tumour suppressor genes as the genetic drivers of cancer cell proliferation.

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Novel biomarker technology for cancer diagnostics

11 September 2020 | By Nikki Withers (Drug Target Review)

A new way of identifying cancer biomarkers has been developed by researchers at Lund University in Sweden. The new technology allows very sensitive, quick and cost-effective identification of cancer biomarkers. Nikki Withers spoke to Professor Carl Borrebaeck, who discusses how the researchers demonstrated the power of combining proteomics with genomics.

news

Urine tests are important in diagnosing myeloma

16 July 2018 | By Drug Target Review

It may be better to examine the urine and the blood of patients suspected of tumours of the plasma cells...

news

Novel label-free assay developed to profile immune response to infection

26 March 2021 | By Victoria Rees (Drug Target Review)

Researchers have created an immune assay that can profile host immune responses to infection and is faster than current methods.

news

Sensitive new assay detects hepatitis B infection in single liver cells and serum

12 April 2018 | By Dr Zara Kassam (Drug Target Review)

Technique measuring the cccDNA marker may allow earlier detection of hepatocellular carcinoma in patients...

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Human iPSC-derived functional cells are revolutionising phenotypic drug discovery and development

4 September 2018 | By Arie Reijerkerk, Farbod Famili, Mieke Doornbos, Stefan Braam

Effective drug discovery and development relies largely on the availability of predictive pre-clinical model systems; the absence of which has contributed to late-stage drug failures and high expenditures. New industry acceptance of human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte assay systems for regulatory safety pharmacology is now resulting in broad…

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Developability assessment of therapeutic antibodies

8 March 2017 | By David Matthews (MRC Technology), Lone Friis (MRC Technology)

Developability assessment is a broad term covering the evaluation of potential therapeutic candidates’ drug-like properties, manufacturability and safety profile. It is important to carry out this assessment as early as possible in the pre-clinical stage development to select the candidate with the most stable profile. This can minimise the risks…

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SOMAscan™ assay: a vital proteomic tool in drug discovery

21 September 2015 | By SomaLogic

The SOMAscan™ assay is a highly multiplexed, sensitive, quantitative and reproducible proteomic tool for discovering biomarkers for drug discovery...

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Review: Biomarkers towards personalised therapy in cancer

2 June 2017 | By Katheryn Begg (King's College London), Mahvash Tavassoli (King's College London)

Since the sequence of the human genome was published some 20 years ago, omics strategies have enabled the generation of detailed molecular signatures of cancers and their subtypes.

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The use of biophysical methods in the hit-to-lead process

22 June 2021 | By Dr Thomas Schubert (2bind)

Optimisation of a hit towards a lead happens in a variety of ways, as a lead needs to meet various criteria such as activity, affinity to the target, selectivity, solubility, permeability or metabolic stability to become a promising candidate. A balance between in vitro profiles and pharmacokinetic attributes has to…

whitepaper

Application note: Faster and more reliable quantification of oligonucleotide interaction with human serum albumin using MST

13 September 2019 | By NanoTemper Technologies

Antisense oligonucleotides are an emerging therapeutic option for treating diseases with known genetic origin.

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Flow cytometry – accelerating neuroscience research

18 September 2017 | By Pushpanathan Muthuirulan

Flow cytometry continues to evolve at a fast pace and provides neuroscientists with the ability to perform many highly-specialised assays simultaneously. It meets the demands of cutting-edge research in neuroscience that has allowed researchers to isolate particular neural cells from heterogeneous population and catalogue its molecular or physical features...

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Antibody fragment technology and avian IgY antibodies: a powerful combination

5 February 2016 | By Carol Harley (IBMC and i3S), Ricardo Vieira-Pires (University of Coimbra)

Recombinant monoclonal antibodies or antibody biologics have been successfully developed as diagnostic and therapeutic agents...