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Technical note: The way to success for GPCR signaling studies – 5 expert tips to boost ERK assay performance

Posted: 12 September 2016 | | No comments yet

This technical note gives you critical insights into on how to run, optimise and validate this ERK assay on your own cellular materials…

The HTRF® Advanced phospho-ERK1/2 kit is a highly sensitive and reliable assay used in the detection of ERK1/2 phosphorylation.

With dozens of data sets, this technical note gives you critical insights into on how to run, optimize and validate this ERK assay on your own cellular materials.

Get the experts’ opinion on:

  • Cell density
  • Effect of serum starvation on signal
  • Stimulation time
  • Best practices in compound dispensing
  • Cell lysis and phosphorylation detection

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