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Application note: Setting up luminometric cell viability assays with the Thermo Scientific multimode microplate reader and Reagent Dispenser

This application note describes how to perform a cell viability assay using the Thermo Scientific Varioskan LUX multimode microplate reader and the Thermo Scientific Multidrop Combi reagent dispenser…

Cell viability was measured using the Promega CellTiter-Glo Luminescent Cell Viability Assay. The procedure and performance of the system and the assay were followed after their optimisation.

Introduction

Cell viability is an important parameter measured in a variety of research areas including drug screening. The CellTiter- Glo Luminescent Cell Viability Assay measures the viability of cells based upon the quantification of cellular ATP levels. The assay relies upon the generation of a ‘glow-type’ luminescent signal which is proportional to the amount of ATP, and thus the number of cells that are present.

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