Cracking cell dynamics with single-cell proteomics and CRISPR
What can pooled CRISPR screening and protein‑level readouts reveal that transcriptomics alone can’t? Explore how single‑cell genomic perturbations, intracellular protein measurements and surface‑protein profiling expose signalling pathways and clarify antibody specificity.
Single-cell transcriptomics can show which genes are active, but it does not always capture the downstream signalling events that influence how cells behave. For researchers working in target discovery and functional genomics, combining genomic perturbation with protein-level measurements can provide a more complete picture of cellular responses.
In this virtual panel, Dr Lukas Badertscher, VP R&D at Myllia, and Dr Majd Ariss, Principal Scientist at Cell Signaling Technology, explore how pooled CRISPR screening can be combined with InTraSeq Single Cell Analysis to measure RNA, intracellular proteins and surface proteins at single-cell resolution.
The speakers will show how linking genetic perturbations with changes in signalling pathways can reveal biological responses that may be missed by transcriptomic measurements alone. They will also discuss how CRISPR gene knockdown can be used to assess antibody specificity in multimodal assays, supporting greater confidence in protein measurements and their interpretation.
Attendees will gain a clearer understanding of what these approaches can offer target discovery and functional genomics, with the opportunity to put their questions directly to the speakers during a live Q&A.
What will you learn?
- Examine how single-cell CRISPR and protein readouts offer a new perspective on single-cell analysis
- Understand how single-cell genomic perturbations and protein quantification can reveal affected signalling pathways at single-cell resolution
- Assess the efficiency of Myllia’s single-cell CRISPR gene knockdown
- Evaluate antibody specificity in single-cell proteomic tools