Application note: Cell line development: accelerating process optimisation

This study demonstrates the ease, simplicity and speed of performing multiple conditions in one experiment using Ambr 15 Cell Culture by assessing five different media compositions on two different clones.

Cell line development (CLD) involves the screening of thousands of clones to find those that are stable, produce high yields of the bioproduct and exhibit desired critical quality attributes (CQAs). Typically, screening and process optimisation activities will be carried out at the small scale in bioreactor cultures to ensure that results translate up to larger bioreactor scales. Performance data is primarily based on cell growth, cell viability, metabolite analysis and product titer and assessed over the duration of the entire culture process.