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Bulletin

Bulletin: Utilising the multiparameter capability of the Ze5 Cell Analyser to monitor T cell exhaustion and effects of immunotherapy

Read this bulletin to learn about a Bio-Rad study where they developed and tested a 16-parameter flow cytometry panel applicable to the interrogation of a range of biologically relevant markers of T cell activation.

Ongoing and future studies of T cell activation using potential immunotherapies rely on the ability to detect the relevant markers that define T cell states. In this study, we developed and tested a 16-parameter flow cytometry panel for the ZE5 Cell Analyser applicable to the interrogation of a range of biologically relevant markers of T cell activation and immunotherapy targets currently being investigated. In lieu of clinical samples, we also developed an in vitro model of T cell exhaustion by activating T cells with beads coated with anti-CD3 and anti-CD28 antibodies (CD3/CD28 activation beads). Nonactivated and exhausted cells were treated with nivolumab, a PD-1 inhibitor, resulting in significant changes in T cell markers detected with the multiparameter panel. Cells were stained and analysed simultaneously for all 16 targets using a ZE5 Cell Analyser configured with five lasers (355, 405, 488, 561, 640 nm), 16 fluorescence detectors, and forward and side scatter.