Whitepaper

Whitepaper: CAR T-Cell analysis with modular antibodies using SpyTag protein ligation

Here Bio-Rad presents the generation of specific anti-CAR T-cell antibodies to show how CAR T-cell analysis benefits from our modular antibody assembly technology using SpyTag-SpyCatcher protein ligation.

Antibodies play a crucial role in the analysis of chimeric antigen receptor (CAR) T-cells.

Particularly of interest is the class of anti-idiotypic antibodies that are directed against the CAR since these enable the specific detection of the modified T-cells using flow cytometry. Here we present the generation of specific anti-CAR T-cell antibodies and demonstrate how CAR T-cell analysis benefits from our modular antibody assembly technology using SpyTag-SpyCatcher protein ligation.