Application note: Quantifying T Cell response in 3D tumour spheroids using advanced flow cytometry workflows.
In addition to assay principles, this application note includes sections on:
Activation marker expression on TILs is greater than on non-infiltrated T cells
Tumor cell type impacts the number and profile of TILs
Activation and exhaustion status of T cells changes throughout spheroid killing
Stimulated T cells shift from early to late stage memory cell development
Anti-HER2 CAR-T killing is specific to HER2 Positive tumor spheroids.
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