Application note

Application note: Dynamic quantification of cell migration in real time

Discover the Incucyte® Scratch Wound Analysis Software Module for automated image-based measurements of cell migration in vitro, using label-free analysis.

Cell migration is a multistep process that plays a fundamental role during essential physiological processes, such as immune cell migration and embryonic development, as well as pathological conditions, such as tumor metastasis. Furthering our understanding of cell migration and cell invasion is important in limiting tumour progression and survival in future therapies. In this application note, we describe the value of the Incucyte® Scratch Wound Cell Migration Assay, for automated image-based measurements of cell migration in vitro and exemplify the ability to visualise and quantify cell migration kinetics.

You’ll learn:

  • The materials and methods
  • Quantification of cell migration
  • Optimisation of migration assay conditions
  • Pharmacological assessment of inhibitors of cell migration
  • Cell cycle and migration


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