Application note

Application note: How to successfully perform live-cell CLEM

Performing live-cell correlative light and electron microscopy (CLEM) with Coral Life.

Coral Life provides a streamlined live-cell CLEM solution for getting insights into the structural changes of cellular components over time. Besides the technical handling described in the workflow, this article provides additional knowledge for a successful experimental run.

For this example, the main interest was the final separation of two mitotic cells, called abscission. After cytokinesis, the two dividing cells are only connected by an intercellular bridge which needs to be resolved. Because of its size, this mechanism can’t be fully understood by live-cell imaging. Therefore, ultrastructural analysis is needed to understand the underlying mechanism of final cell separation.