A selective FACS-enrichment was performed via the enrichment mode of Namocell Single Cell Dispenser to enrich for high titer CHO cells.

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Cell line generation for monoclonal biotherapeutic workflows use FACS instruments to screen clones from transfected pools. While able to achieve cell isolation, the technology relies on high pressure (>20 psi) sorting, often resulting in low cell viability and high dead volume. In this application note, a Namocell single cell dispenser isolated high titer CHO cells using less than 2 psi gentle sorting pressure to preserve cell viability.

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Asset 1: Enrichment of high titer CHO clones

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