Application note

Application note: Real-time kinetic analysis of Akt activity

Cell signalling through the Akt pathway controls numerous key cellular functions including proliferation, survival, and migration.

The ability to measure real-time Akt activation in living cells will provide valuable information on the involvement of Akt across multiple fields of research including oncology, neuroscience and inflammatory disease research. This application note includes detail on cell signalling through the Akt pathway, profiling Akt activity response to inhibitors and a focus on growth factors.

You will learn:

  • Assay principle and quantification
  • Direct and indirect inhibition of Akt
  • Targeting upstream regulators of Akt activity
  • Regulation of Akt activity in inflammation response
  • Akt activity regulates cell function.

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