Utilizing Advanced High-Throughput Flow Cytometry to Quantify Direct and Competitive Live Cell Antibody Binding
Learn how to enable high-throughput measurement of antibody binding activity with this new application note from Sartorius!
Specific binding of antibody to the target antigen is one of many key attributes that must be evaluated during development, alongside characteristics such as Fc function, stability and post-translational modifications.
Register your details to access this exclusive application note to discover:
Issues with conventional techniques for evaluating antibody binding
Two simple assays that utilise the iQue® Advanced Flow Cytometry Platform and validated reagents to measure binding of unlabeled therapeutic mAbs to your target on live cells
Method and workflow for a direct binding assay and competitive binding assay
Assessment of mAb binding and specificity in both adherent and suspension cell models
Comparing biosimilar binding to live target cells
Measuring antibody binding to a range of target cells in a single well
High-throughput screening of binding and thermal stability in 384-well format
Download now to learn easy-to-follow protocols for measuring antibody binding.
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