Application note

Application note: Direct and Competitive Live Cell Antibody Binding

Utilizing Advanced High-Throughput Flow Cytometry to Quantify Direct and Competitive Live Cell Antibody Binding

Learn how to enable high-throughput measurement of antibody binding activity.

The direct binding workflow provides robust quantification of binding to native protein on live cells, with simple generation of pharmacological readouts, such as EC₅₀ values, using a single and comprehensive software package.

The competition binding assay provides a streamlined workflow to assess antibodies that bind the same epitope on the target antigen.

Download now to learn easy-to-follow protocols for measuring antibody binding.