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Assays

 

An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.

Application note: GABAA Receptors

11 December 2018 | By

SB Drug Discovery shows the validation of fluorescence and automated electrophysiology assays designed to assess agonists, antagonists and allosteric modulators of these receptors, culminating in a high-throughput electrophysiology assay suited to assessing multiple GABAA receptor subtypes on a single assay plate.

Expert view: Three-dimensional cell cultures as predictive tools in early drug discovery

6 December 2018 | By

Three-dimensional cell cultures (spheroids, organoids) are becoming widely used as a new predictive tool in early drug discovery. The use of 3D cell cultures is believed to provide a more physiologically relevant response than monolayer (2D) cell cultures because they closely mimic the extracellular matrix and cell-cell interactions that occur…

A high-throughput approach for measuring intracellular ATP levels to access compound-mediated cellular toxicity

30 November 2018 | By , , ,

Many drugs fail in clinical trials due to toxicity-related issues; therefore, drug-mediated cellular toxicity should be determined at an early stage in the drug discovery value chain. There are many assay systems that can be utilised to measure cellular toxicity. In this article we demonstrate that intracellular ATP concentrations are…

Application note: Production of human induced pluripotent stem cell-derived cortical neurospheres in the DASbox® Mini Bioreactor System

29 November 2018 | By

There is substantial interest in the scientific community in using human induced pluripotent stem cell (hiPSC)-derived neural cells to study basic mechanisms of brain development, neuronal function, and drug-induced effects. In vitro-generated brain organoids are promising models, because they show a remarkable degree of maturationand can be kept in culture…

Application note: Sensitive fluorometric quantification of DNA in microplate format

29 November 2018 | By

This technical note demonstrates the feasibility of using the Thermo Scientific™Fluoroskan™ or Fluoroskan FL instruments with the Invitrogen™ Quant-iT™ dsDNAhigh-sensitivity assays. Sensitive and specific quantification of double-stranded DNA is carried out in microplate format. This is part of a series of technical notes aiming to help customers choose the best…

Application note: A novel solution to expedite antibody discovery

29 November 2018 | By

With breakthroughs in molecular engineering and antibody humanisation, monoclonal antibodies (mAb) are one of the fastest-growing classes of biopharmaceuticals for multiple clinical indications including cancer, cardiovascular disease, autoimmune disorders and infectious disease. Most therapeutic antibody candidates are initially generated using hybridoma technology or primary B-cell screening after antigen immunisation.