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Assays

 

An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.

High-content imaging: challenges of the 3D world

17 June 2019 | By ,

The potential of three-dimensional cell culture models is now well-recognised by scientists; however, there are still some challenges. Margaritha Mysior and Jeremy Simpson address some of the common challenges of working with spheroids and organoid models – including how they are developed and how to obtain high-resolution, high-content images and…

Phenotypic profiling in drug discovery

5 June 2019 | By , ,

Image-based phenotypic profiling of small molecules has been used for identification and characterisation of small molecules in drug discovery and can provide important insights into their mechanisms of action (MOA). This article discusses the use of phenotypic profiling in drug discovery and how machine-learning algorithms could help improve objectivity, processing…

AI-driven automated chemistry as a tool to accelerate drug discovery

15 May 2019 | By ,

Typically, early drug discovery campaigns start with the screening of chemical libraries to identify candidate chemotypes modulating a particular target and/or phenotype. Success of the primary screening depends on multiple factors related to both biology and chemistry. These include the target’s druggability, sensitivity and specificity of assay system, composition and…

The importance of characterising chemical starting points of drugs using appropriate in vitro ADME-toxicity assays

21 March 2019 | By ,

Over the past 30 years, one strategy the pharmaceutical industry has adopted in the drug discovery process has been to “fail early, fail often”.1,2 As most molecules in the early stages of drug discovery will have sub-optimal characteristics, significant modification is necessary to improve their properties.