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Application note: Orthogonal approaches for studying bacterial biofilms using photometric detection

Posted: 19 October 2018 | | No comments yet

This application note aims to demonstrate the benefits of using orthogonal approaches for studying Staphylococcus aureus biofilms—in particular, their susceptibility to antimicrobial agents…

This is exemplified by performing a single adherence assay based on crystal violet staining, which allows both quantification of biofilm biomass and imaging of the attached cells. In the workflow presented here, the measurement of the photometric signal from the biomass-bound crystal violet dye was performed with a Thermo Scientific™ Multiskan™ Sky Microplate Spectrophotometer, which was complemented with the brightfield imaging of the microbial attachment pattern using an Invitrogen™ EVOS™ FL Auto Imaging System.

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