ebook: Cytokine and protein – User’s guide book
Many cell lines require the addition of specific growth factors to their culture media even if the media contains serum.
Most intracellular proteases are found in lysosomes, sequestered from other cellular components. The separation is important so they do not destroy proteins that are vital to the cell’s function. When you make cell lysates, though, you disrupt lysosome structure and give the proteases access to everything in the lysate.