A high-throughput approach for measuring intracellular ATP levels to access compound-mediated cellular toxicity
Posted: 30 November 2018 | Maria Kuzikov (Fraunhofer-IME SP), Oliver Keminer (Fraunhofer Institute for Molecular Biology and Applied Ecology), Salma Hachim (Fraunhofer Institute for Molecular Biology and Applied Ecology), Sheraz Gul (Fraunhofer-IME SP) | No comments yet
Many drugs fail in clinical trials due to toxicity-related issues; therefore, drug-mediated cellular toxicity should be determined at an early stage in the drug discovery value chain. There are many assay systems that can be utilised to measure cellular toxicity. In this article we demonstrate that intracellular ATP concentrations are a sensitive method with which to determine cellular toxicity and, as a validation exercise, we report a screening compatible cellular toxicity assay protocol. More importantly, we report the output of a Proof-of-Concept screen that made use of this assay against a known drug library and the MCF-7 cell-line. The results will be beneficial for those engaged in drug repurposing.