Abcam MMP Activity Assay Kit (Fluorometric – Red, ab112147)

MMP Activity Assay Kit (Fluorometric – Red, ab112147) uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate…

Manufactured by Abcam 

MMP Activity Assay Kit (Fluorometric – Red, ab112147) uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of one part is quenched by the other. Upon cleavage into two separate fragments by MMPs, the fluorescence is recovered. The kit is designed to check the general activity of a MMP enzyme and to screen MMP inhibitors when a purified MMP enzyme is used.

With excellent fluorescence quantum yield and longer wavelength, this substrate shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than the commonly used EDANS/Dabcyl FRET substrate. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The pH-independent fluorescence makes the assay reading available for the whole physiological pH range. The high photostability of this FRET peptide provides a useful imaging probe. Many labs have used this kit for the high throughput screening of MMP inhibitors as potential anticancer drug candidates.

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