Application note: KingFisher sample purification system

Next generation sequencing sample preparation on the KingFisher platform…

In this application note, Thermo Scientific discuss how their KingFisher instruments are well suited for various NGS sample preparation steps.

Next generation sequencing (hereafter NGS) refers to a high-throughput nucleic acid sequencing technology that is significantly faster and more cost effective compared to former sequencing methods. The technology has revolutionised molecular biology and genomics research and holds great promise for the future of personalised medicine.

Thermo Scientific™ KingFisher™ instruments employ magnetic particle technology for biomolecule purification. Instrument function is based on the transfer of magnetic bead bound samples through successive sets of solutions that are necessary for the extraction protocol. KingFishers are well suited for various NGS sample preparation steps. Since only the bead-sample complex is transferred, the amount of carryover wash buffers or other impurities remains minimal.

KingFisher ready-made protocols with easy-to-use, intuitive BindIt software allow users to quickly and easily set up new protocols or make modifications to existing scripts.

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