whitepaper

Application Note: Full-length, single-cell RNA-seq with the SMARTer™ ICELL8® Single-Cell System

Posted: 6 September 2018 | Takara Bio | No comments yet

The data presented in this application note demonstrates how SMARTer ICELL8 Single-Cell System can be used to generate high-quality RNA-seq libraries that provide full-length transcript sequence information from hundreds of single cells in parallel…

Our method for full‑length single-cell RNA-seq using the SMARTer ICELL8 system enables users to perform all reactions required for Illumina NGS library preparation in the SMARTer ICELL8 chip, including cDNA synthesis and PCR amplification, so that the output from the chip is a sequencing-ready library. Along with incorporating SMART template-switching technology to ensure production of high-quality cDNA, our method enables various single-cell analyses to be performed with high sensitivity and reproducibility.

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