Application note: Measure cancer cell viability using a homogeneous, stable luminescence assay

Posted: 29 November 2019 | | No comments yet

Learn how to screen for cell viability with the CellTiter-Glo assay on the SpectraMax® iD5 Multi-Mode Microplate Reader.

Luminescent cell viability assays use the firefly luciferase reaction as a way to determine the relative numbers of living cells under different treatments or experimental conditions. Metabolically active cells produce ATP, which is required by the luciferase reaction. When ATP is the limiting component in the reaction, the amount of light produced is proportional to, and serves as the readout for, the number of viable cells.

In this application note, scientists from Molecular Devices validated the performance of Promega’s CellTiter-Glo 2.0 Cell Viability Assay on the SpectraMax® iD5 Multi-Mode Microplate Reader. Both ATP and viable cells were used to generate standard curves demonstrating the sensitivity and linearity of the assay.

  • Highly sensitive luminescence detection down to 20 cells per well
  • Easy correlation of cell number and ATP content
  • Convenient single-reagent workflow



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