Advertorial

Expert view: Engineered antibodies: semi-rational design approach for faster optimisation

Posted: 13 September 2019 | | No comments yet

A significant hurdle in optimising antibody therapeutics is the screening of successive rounds of large libraries of mutant variants in order to recognise the ideal candidate.

Here, we discuss how a semi-rational design approach can create diverse mutant libraries, significantly reducing the overall screening effort and leading to faster identification of the optimal drug candidate.

Antibody-based drug candidates require additional engineering to improve stability, solubility and target affinity, as well as decrease immunogenicity. To identify the ideal optimised drug candidate, antibody engineers use a technique called directed evolution that requires high-throughput approaches to create and screen a large library of mutant variants. Mutant libraries are generated in large scales using polymerase chain reaction (PCR), cloned into expression vectors and screened via phage or yeast display.

    To read this Expert View in full, please complete the form below. By clicking submit you confirm that you accept our terms and conditions and privacy policy.


    *


    *


    *


    *


    *


    *


    *


    *

    This content is provided to you for free thanks to the kind support of our sponsor: GenScript

    Related organisations