Poster note: Target discovery program in systemic sclerosis
Novel candidate drug targets for systemic scleroderma were identified using RNAi in phenotypic assays in scleroderma patient-derived fibroblasts…
This poster outlines a study to identify and validate novel candidate drug targets using RNAi in phenotypic assays in systemic sclerosis patient-derived fibroblasts.
A high throughput primary screen measuring the reversion of myofibroblasts was developed to screen Charles River’s SilenceSelect® adenoviral shRNA library (~21,000 shRNAs screened). Inhibition of aSMA mRNA expression was measured via fluorescence in situ hybridization (FISH), and ~550 shRNAs were selected for rescreen. Follow on 2D and 3D validation assays including gel contractility, impedance and skin equivalent (SE) are described.
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