Application note: Real-time detection of Gs and Gi Signalling in living cells
G-protein coupled receptors (GPCRs) are predominant drug targets. Therefore, monitoring GPCR activity upon compound addition in a cellular context is required. To this end, Montana Molecular developed biosensors that detect G-protein-driven cAMP generation. The sensor consists of a specific cAMP binding domain and a fluorescent label. It changes its fluorescence intensity if the second messenger cAMP is bound.
The rest of this content is restricted - login or subscribe free to access
Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.
Why subscribe? Join our growing community of thousands of industry professionals and gain access to:
- quarterly issues in print and/or digital format
- case studies, whitepapers, webinars and industry-leading content
- breaking news and features
- our extensive online archive of thousands of articles and years of past issues
- ...And it's all free!
Related content from this organisation
- Application note: Screening for optimal association and dissociation rates of ligands using a live cell binding assay
- Expert view: Promising methods in kinase screening
- Screening In-Depth Focus 2019
- Under the microscope: Improving hit discovery efficiency at Europe´s leading screening centre
- Webinar highlights: Cell-based and biochemical high-throughput screening in 1536-well plates