Application note: Tools and protocols for high-content imaging and analysis

Integrating hardware, software and fluorescent labels for optimised HCA assay development.

High-content imaging and analysis transforms fluorescence microscopy into a high-throughput, quantitative tool for investigating spatial and temporal aspects of cell biology. Automation – not only of the image acquisition but also of the analysis – allows millions of cells to be analysed and reveals the heterogeneity of responses that exist within cell populations. These cellular responses can then be assessed across a range of manipulations, whether they are genome-wide screens or small-molecule library analyses.

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