Application note: A Simoa ™ Pharmacokinetic Bridging Assay
Pharmacokinetics (PK) refers to the movement of a drug through the body. The distribution, absorption, metabolism, and excretion of a drug are all important pharmacokinetic factors to be considered in the development of therapeutics.
The ability to detect and quantify the concentration of a therapeutic molecule with high sensitivity may be necessary for effective pharmacokinetic studies, especially if the drug is administered at a low dose. Here we describe a proof of concept assay to demonstrate the Simoa platform as a tool for measuring concentration of the biotherapeutic IgG Adalimumab (Humira®). A sandwich ELISA was created using anti Adalimumab antibodies as capture and detection.To compare performance, equivalent assays were run with the Simoa platform and a standard plate based ELISA.
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