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An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.
30 October 2018 | By BMG Labtech GmbH
In this webinar, we presented two examples of HTS in 1536-well plates, a screen for protein-protein interactions and a cell-based luciferase reporter assay.
This application note aims to demonstrate the benefits of using orthogonal approaches for studying Staphylococcus aureus biofilms—in particular, their susceptibility to antimicrobial agents...
High throughput screening is mostly done in pharmaceutical companies that test whole libraries of compounds. Reducing the assay volume will reduce operating costs. At the same time it is necessary to prove that the smaller volume will not have a negative impact on assay sensitivity and reliability.
Due to the crucial role of G protein-coupled receptors (GPCRs) in mediating cellular responses to external stimuli, these receptors have been and will remain a prime focus for medical research and the pharmaceutical industry for many years to come. Their importance is especially highlighted by the statistic that 30-50% of…
This webinar shines a spotlight on cell-based and biochemical high-throughput screening in 1536 well plates. Supported by BMG LABTECH, the webinar will take place on 30 October at 4:00pm (GMT).
This webinar, held on 28 June 2018, illustrated the optimisation of hits derived from a target-agnostic phenotypic screen, using a strategy based on combining bioactivity profiling and reference compound characterisation. Keynote speaker Arsenio Nueda, Head of Molecular Biology at Almirall, responded as follows to questions posed during the webinar.
Flow cytometry has played a key role in drug development, largely due to its ability to measure up to twenty parameters on single cells or particles suspended in a fluid stream. Typically, cells are labelled with fluorescent markers and passed through a series of lasers, after which the resultant fluorescent…
This Assays In-Depth Focus looks at applications of high content screening in autophagy, and complex biology for the precision medicine era.
Stem cell-based technologies lay the basis for pioneering approaches in drug screening, toxicology testing, and regenerative medicine. The constant supply of high cell numbers in consistent quality needs scalable culture systems and standardised process conditions.
Growing evidence reveals that using single immunotherapies to treat oncological disorders may lead to cancer-killing T-cell overdrive,initiating life-threatening autoimmune reactions including cytokinestorm; also known as hypercytokinemia or Cytokine Release Syndrome(CRS). Recently, checkpoint blockade using anti-programmed cell death1 (anti-PD-1) inhibitors became a prevalent approach for stimulatinganti-tumour immune system.
Assessing targets that are unable – or rather, extremely difficult – to reach pharmacologically, has prevented researchers from achieving desired clinical successes, most notably in the realm of cancer research. However, many advances are being made to shedlight on these difficult yet desirable target areas.
Live-cell analysis is now a well-established method that is part of the everyday cell biologist’s armoury for understanding cell biology. For over a decade, the functionality, throughput, and ease of use offered by real-time live-cell analysis has provided a platform for accurate and reproducible cell biology analysis in multiple biomedical…
Complex biology is a discipline acknowledging that performing biological experiments in vitro should take account of the complexity of the biological context.1 While this may be a noble aim, it has proven difficult to incorporate these elements into the drug discovery process, especially at the high-throughput screening (HTS) stage.2
Autophagy is an important process to maintain cellular homeostasis and function.1 Basal levels of autophagy are essential for most cells to remove unwanted protein aggregates and damaged organelles in order to prevent diseases.2 However, sometimes cells are unable to maintain physiological stability as a consequence of altered autophagy, which leads…
In this application note, we show how the signal of luciferase and fluorophore were acquired simultaneously with the PHERAstar microplate reader...
