Cell-based and biochemical high-throughput screening in 1536-well plates
ABOUT THIS WEBINAR
The Small Molecule Screening Facility (SMSF) at the University of Wisconsin Madison is a comprehensive high-throughput screening (HTS) centre that offers services to academic and industry clients worldwide. The facility has an in-house chemical library of >450,000 compounds that are available for screening to our customers.
In this webinar, speakers Dr. Gene Ananiev, Dr. Carl Peters and Dr. Iain Russell discussed two projects. In one of the projects they demonstrated the creation of a robust FMR1-Nluc reporter hiPSC line by knocking in a Nano luciferase (Nluc) gene into the endogenous human FMR1 gene using the CRISPR/Cas9 genome editing method. We confirmed that luciferase activities faithfully report FMR1 gene expression levels and showed that neural progenitor cells derived from this line could be optimised for high-throughput screening.
The second project focused on how antibiotic-resistant bacterial infections are increasingly prevalent worldwide, and how there is an urgent need for novel classes of antibiotics capable of overcoming existing resistance mechanisms. In this project, nine potent and selective inhibitors produced concentration-response curves with IC50 values of <40 μM, and two compounds were observed to directly bind to PriA, demonstrating the success of this screen strategy.
Here speakers presented two examples of HTS in 1536-well plates, a screen for protein-protein interactions and a cell-based luciferase reporter assay. Using a miniaturised AlphaScreen we were able to drastically reduce cost and increase throughput for protein-protein interaction HTS. For cell-based HTS they discussed screening for activators of gene-expression using iPSC derived, neural progenitor cells, engineered with a Nano-Luciferase reporter. They presented methodology for assay miniaturisation and optimisation in 1536-well format. We will discuss the impact of the above on academic HTS endeavours at our facility.
Dr Gene E. Ananiev, PhD, Core Facility Manager at University of Wisconsin Madison, Carbone Cancer Center
Dr. Gene E. Ananiev, graduated from Worcester Polytechnic Institute with a degree in Biology. He received his Ph.D. in Cellular and molecular biology from UW Madison, working with Prof David. C. Schwartz, on developing high throughput methods in genomics. As a postdoc he worked on assay development for drug discovery using iPS cell derived neurons with Prof Qiang Chang. In 2012 Gene took a position as a research scientist and manager for High Throughput Screening at the UW Madison small molecule screening facility. In the past five years, the focus of the facility has been the development of cell based and biochemical assay in 1536 well format.
Dr Carl Peters, PhD, Senior Applications Scientist at BMG LABTECH
Dr. Carl Peters is a Microplate Reader Senior Application Scientist at BMG LABTECH. He obtained a PhD in Cell and Molecular Biology from Northwestern University while studying Protein Kinase C signaling. He also has a B.S. in Biology from Hastings College. Prior to BMG LABTECH, he was an adjunct professor of Biology at Roosevelt University and subsequently a Clinical Assistant Professor in the School of Molecular Biosciences at Washington State University.
Dr Iain Russell, Senior Product Manager, Labcyte, Inc.
Dr. Iain Russell has over 15 years of experience in the Life Sciences industry and the genomics field. Iain manages Labcyte’s Echo® Liquid Handler product line, a series of instruments that enables the touchless transfer of nanoliter liquid volumes with extreme accuracy and precision. Prior to joining Labcyte, Iain managed a variety of technologies including microarray- and qPCR-related instruments and consumables.