Application Note: Fluorescence Polarization based assay for rapid, precise, high-throughput measurement of IgG & Fc containing derivatives
The accurate, rapid and high-throughput measurement of IgG is essential in the development and manufacture of most therapeutic antibodies…
Monoclonal antibodies are becoming increasingly dominant in biopharmaceuticals, where a vast number of samples must be screened for the development of each potential therapeutic.
Here we report the development of a novel, rapid, and simple fluorescence polarization based assay for high-throughput titer measurement of IgG and Fc-containing derivatives. It uses fluorescently labeled protein G that binds IgG. Upon binding, the depolarization of emitted light decreases, this can be detected to report on the presence of IgG. The CLARIOstar®, PHERAstar® and POLARstar® exhibit excellent assay quality when used with the ValitaTITER assay, which enables a high-throughput, simple, precise method for quantification of IgG. Moreover, the assay can be performed using sample straight cell culture supernatant, which means there are no complex sample preparation steps.
This application note is restricted - login or subscribe free to access
Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.
Why subscribe? Join our growing community of thousands of industry professionals and gain access to:
- quarterly issues in print and/or digital format
- case studies, whitepapers, webinars and industry-leading content
- breaking news and features
- our extensive online archive of thousands of articles and years of past issues
- ...And it's all free!
Related content from this organisation
- Application note: Screening for optimal association and dissociation rates of ligands using a live cell binding assay
- Expert view: Promising methods in kinase screening
- Screening In-Depth Focus 2019
- Under the microscope: Improving hit discovery efficiency at Europe´s leading screening centre
- Webinar highlights: Cell-based and biochemical high-throughput screening in 1536-well plates