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Technical note: 3D volumetric analysis of luminal spaces inside cysts or organoids

Posted: 6 June 2018 | | No comments yet

High-content assays using 3D objects, such as cysts or organoids, can be challenging from the perspectives of both image acquisition and image analysis.

In this technical note, we describe how to image and analyse epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS high-content analysis system. 

We address:

  • How to reduce the loss of image resolution, as you image deeper into the sample
  • How to increase throughput for 3D imaging through shorter exposure times
  • How to enable 3D segmentation and analysis for your 3D high-content assays
  • How to decrease the number of data transfer steps between software packages.

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