Whitepaper: Neural Cell Culturing Guide
The advent of in vitro culturing of neural cells has been central to driving our understanding of the nervous system.
The complexity of neural tissue makes it difficult to study the cellular and molecular mechanisms underlying neural functioning. Cell culture systems have given researchers the means to study the nervous system at this level. This guide provides an introduction to the techniques and protocols for successfully isolating and culturing specific cell types from the rodent central and peripheral nervous systems, as well as the expansion of mouse and rat cortical stem cells.
Contributors include Bio-Techne scientists from our bioassay and stem cell divisions, who have experience in culturing and maintaining multiple types of neural cells. As with every new procedure, these protocols may need to be optimized for your experiment. This guide also presents the industry’s premiere media supplements, adhesion substrates, bioactive proteins, verification antibodies, and neuromodulatory compounds from the Bio-Techne brands R&D Systems, Tocris, and Novus Biologicals for neural cell culture research.