whitepaper

App Note: Easy Transfer of Your Traditional Western Blot to Wes

Posted: 10 October 2015 |

Wes™ builds on over a decade of Simple Western development and makes running a Western even more simple…

The Western blot has been around in essentially the same form since the late 1970s. A proteomics workhorse, Westerns are a key part of protein analysis in many labs.

That said, the continuous manual intervention to run a Western blot is labor intensive and introduces many opportunities for error. Wes™ builds on over a decade of Simple Western development and makes running a Western even more simple. Wes eliminates all the repetitive processing by automating all manual processes. Loading the “gel”, monitoring the separation, “blotting”, blocking, antibody incubations, washing, detection and even analysis of data is all done automatically. Assay kits come with a pre-filled microplate, so the only reagents you need to pipette are your samples, your antibodies, and your molecular weight ladder, everything else needed to run the assay is already in the plate. Wes also uses a capillary cartridge that is fully self-contained and stores all assay waste inside. At the end of the run, just toss the used plate and cartridge and you’re done. The Compass software used to run Wes also allows you to run standard curves and back-calculate protein quantitation values for unknown samples. Wes not only gives you a fully automated instrument to run your Westerns on for up to 24 samples (plus a molecular weight ladder), he also gives you more from that data than ever before.

This application note is restricted - login or subscribe free to access

drug target review 1 2019 coverThank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • quarterly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here

 


Send this to a friend