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ProteinSimple Wes

Simple Western™ system that lets you separate and analyse proteins by size from 2-440 kDa with just 3 µL of sample…

Simple Westerns just got even simpler

Wes™ lets you separate and analyse proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don’t wait to discover, get going today with Wes!

TECHNOLOGY & APPLICATIONS

SIMPLE WESTERN ASSAYS ON WES

.  Total Protein detection (left) and Immune detection (right)

FIGURE 1.  Total Protein detection (left) and Immune detection (right) of decreasing concentrations of DNAK in Hela lysate and negative controls (15, 7.5 and 3.75 µg/mL in the total protein assay; 0.015, 0.0075 and 0.00375 µg/mL in the immunoassay).

GST-labeled AKT was used to generate a standard curve for quantitation of endogenous AKT. AKT-GST was spiked into the Jurkat lysate at decreasing concentrations (250—0 pg/µL) and both the labeled and endogenous proteins were detected using an AKT1 monoclonal antibody

FIGURE 2.  GST-labeled AKT was used to generate a standard curve for quantitation of endogenous AKT. AKT-GST was spiked into the Jurkat lysate at decreasing concentrations (250—0 pg/µL) and both the labeled and endogenous proteins were detected using an AKT1 monoclonal antibody. Linear regression analysis was performed and the endogenous concentration of AKT in the samples was calculated as 21 pg/µL.

Simple Western 90 kDa System Control and endogenous C-Abl, BCR-Abl, and CBP in K562 cells detected simultaneously by Wes.

FIGURE 3.  Simple Western 90 kDa System Control and endogenous C-Abl, BCR-Abl, and CBP in K562 cells detected simultaneously by Wes.

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