Establishing Alpha CETSA HT in primary screening

ABOUT THIS WEBINAR
High-throughput screening (HTS) cascades have evolved to ensure that high quality hits can be identified from large screening collections. Traditionally, most primary screens focus on the identification of modulators of catalytically active sites, while target engagement assays are placed further down the cascade. The cellular thermal shift assay (CETSA®) is a physiologically relevant assay to detect endogenous protein levels. It directly identifies target engagement in a cellular environment, removing challenging and time-consuming protein or cell line generation steps and it can be performed in an HTS compatible isothermal microwell setup.
This could offer a fast-tracked starting point into the hit discovery process and has an attractive outlook of finding new chemistry that enables both classic functional modulators as well as potential novel PROTACS warheads. In this webinar you will see how Vertex Pharmaceuticals and AstraZeneca are exploring the power of CETSA®-HT, in conjunction with Alpha technology for detection, for larger scale primary HTS campaigns in close collaboration with Pelago BioScience and PerkinElmer.
Learning outcomes of this webinar:
Biomarkers aren’t just supporting drug discovery – they’re driving it
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- Using CETSA® HT for compound screening and hit confirmation
- Applying CETSA® HT on targets that are lacking chemical starting points
- Overcoming challenges when using CETSA® HT in high-throughput screening
- How CETSA® HT enables protein degradation focused drug discovery projects, such as PROTACs.
KEYNOTE SPEAKERS
Julie Alexandre, Senior Research Scientist, Vertex Pharmaceutical
Kirsten Tschapalda, Senior Research Scientist, AstraZeneca
Related topics
Antibodies, Assays, Biomarkers, Disease Research, Hit-to-Lead, Lab Automation, Label-Free, Personalised Medicine, Screening, Targets, Toxicology
Related organisations
Pelago Bioscience, PerkinElmer