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Poster: Expansion of human induced pluripotent stem cells in scalable stirred suspension culture

Posted: 12 January 2018 | Merck | No comments yet

Expansion of hiPSCs as aggregates in stirred suspension…

Background

  •  The cell concentrations recommended for clinical scale tissue engineering warrant the use of systems that are capable of generating large batches of human induced pluripotent
    stem cells (hiPSCs) in a controlled manner.
  • Stirred suspension culture is a scalable method that has been used to successfully manufacture vaccines and therapeutic cells for numerous indications.
  • Prior studies have shown the ability to culture hiPSCs in stirred suspension culture, however the culture vessels have mostly been limited to small scale spinner flasks.

Objective

  •  To grow hiPSCs as aggregates in 125mL spinner flasks and scale up to a 1L culture in a single-use bioreactor.

 

If you are viewing this poster in the USA or Canada, please click here to view the MilliporeSigma version.

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada

 

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