Case Study: Characterisation of hiPSCs by Phenotypic Screening
Case study detailing a simple, highly reproducible phenotypic screen to characterise human induced pluripotent stem cells…
Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics.
The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterisation and data analysis tools.
This case study details a phenotypic screen developed by HipSci to characterise human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact
It describes how:
- high-content analysis enables image-based phenotyping of human induced pluripotent stem cells
- to distinguish single cells from cells in clumps or colonies
- secondary data analysis tools allow a better understanding of multiparametric datasets such as phenotypic profiles.
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