Tech note: Detection of immune checkpoint markers in peripheral blood mononuclear cell lysates with AlphaLISA

Quickly and easily quantitate immune checkpoint markers using a fraction of the sample volume required by traditional ELISAs…

In this technical note we show how AlphaLISA can be used to detect and quantify additional immune checkpoint molecules within the PBMC population with and without activation using Dynabeads ®


  • The benefits of AlphaLISA over traditional ELISAs for the detection of immune checkpoint proteins, including a drastic reduction is sample consumption
  • How ATPliteTM 1step can be used to quickly and easily assess cellular proliferation
  • How the use of the two technologies together provides deeper insight into the mechanisms behind upregulation

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