Application note: automated cell transfection and reporter gene assays
Reporter gene assays have greatly helped our understanding of gene regulation and signal transduction in vitro and in vivo.
They are widely used in molecular and cell biology studies, as well as in biomedical and pharmaceutical research and development. A typical reporter gene assay includes cell transfection of a reporter gene sequence cloned into an expression vector and the determination of the gene expression level by measuring the reporter activity. We demonstrate here how a reporter gene assay can be automated using two Biomek NXP workstations, yielding accurate and highly reproducible measurements of reporter activity.
Please complete the form below in order to download the Application Note:
Related content from this organisation
- Expert view: High-throughput screening – make an impact by removing barriers
- Application note: automated cell transfection and reporter gene assays
- High throughput screening for deep mining of antibody repertoires, Dr. Stefan Moese, Novartis, Switzerland
- Expert view: Tools for compliance with 21 CFR Part 11
- Flow Cytometry In-Depth Focus 2018