App Note: High Molecular Weight Protein Analysis Made Simple
Posted: 9 October 2015 | ProteinSimple
Wes now analyzes proteins up to 440 kDa with the same Simple Western speed and data quality you’ve come to enjoy…
We all know analyzing a big protein using a traditional Western blot is easier said than done.
First, the low percentage gel you have to use rips easily when you move it to the transfer apparatus. Next, there’s the daunting task of optimizing transfer conditions to efficiently migrate your protein to the membrane. Plus it’s also fairly common to have to transfer your protein overnight instead of the usual hour, increasing your time to results with a traditional Western blot from two days to three. Then at the end of those three long days, all you’re left with is a method that’ll give you sub-par reproducibility at best.
Well, you’ll never need to worry about any of that again! Wes now analyzes proteins up to 440 kDa with the same Simple Western speed and data quality you’ve come to enjoy. And thanks to his proprietary capture chemistry, you’ll never have to wonder if your proteins transferred efficiently either. To top that off, you can do all of this with just 30 minutes of hands-on-time and get results in less than three hours.
And while we’ll primarily discuss Wes data in this application note, everything you’ll see here can also be done on Sally Sue and Peggy Sue as well. So for those of you who really need a high-throughput solution — don’t worry, that’s available too!
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