Application note: Evaluation of different ion-pairing reagents for LC/UV and LC/MS analysis of oligonucleotides

Posted: 25 January 2021 | | 1 comment

The effect of various ion pairing reagents (amines and acidic counterions) on the LC/UV and LC/MS analysis of oligonucleotides (ONs) has been studied.

This application note shows that there are numerous variables available for optimisation of the analytical conditions for oligonucleotides.  The analyses were carried out on an Agilent 1290 Infinity II LC System with UV detection by DAD and an Agilent 6530 LC/Q-TOF.

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