Application note: Effectiveness of CRISPR‑Cas9 using pools of synthetic crRNAs in high-content analysis screening experiments
Chemical synthesis of guide RNAs for CRISPR-Cas9 gene editing enables accurate and rapid production of CRISPR libraries and screening in an arrayed, one-gene-per-well fashion.
Gene knockout using the CRISPR-Cas9 system has emerged as a powerful technology for loss-of-function screening. Although screening using pooled lentiviral sgRNA libraries is a powerful way to discover gene function, arrayed screening expands the types of phenotypic readouts from simple population enrichment or depletion to complex multiparametric high-content imaging and morphological assays. Here, four pooled crRNA to the same target are shown to provide robust functional knockout in arrayed CRISPR screening experiments.
Learn more about how crRNA pools can work successfully in phenotypic screening experiments and reduce costs by downloading the application note below.
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- Application note: Effectiveness of CRISPR‑Cas9 using pools of synthetic crRNAs in high-content analysis screening experiments