Scientific poster: Developing protein: Protein Interaction (PPI) assays with AlphaLISA and LANCE TR-FRET

In this scientific poster, PerkinElmer demonstrates the ease and utility of using AlphaLISA® and LANCE® TR-FRET homogenous assay forms for studying PPIs.

Protein: protein interactions (PPIs) are central to the molecular processes that take place within a cell, as the function of any given protein is often regulated by other proteins. In recent years, modulation of PPIs has received increased attention in the scientific community as a whole, and specifically within the realm of drug discovery. PPIs are being studied as therapeutic targets in a variety of diseases including cancer, cardiovascular disease, and even HIV.

Currently, there are a variety of methods used to detect PPIs, each with its own strengths and weaknesses. This is especially true in regards to sensitivity and specificity. However, the ideal assay is a cell-based, homogenous assay that maintains both its specificity and sensitivity.

Here we show that both the AlphaLISA and LANCE TR-FRET technologies allow for the development of protein: protein interaction assays in cell-based, homogenous no-wash formats suitable to HTS applications.

This scientific poster is restricted - login or subscribe free to access

Drug target review issue 1 2018 coverThank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • quarterly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here


Send this to a friend