Scientific poster: Developing protein: Protein Interaction (PPI) assays with AlphaLISA and LANCE TR-FRET
In this scientific poster, PerkinElmer demonstrates the ease and utility of using AlphaLISA® and LANCE® TR-FRET homogenous assay forms for studying PPIs.
Protein: protein interactions (PPIs) are central to the molecular processes that take place within a cell, as the function of any given protein is often regulated by other proteins. In recent years, modulation of PPIs has received increased attention in the scientific community as a whole, and specifically within the realm of drug discovery. PPIs are being studied as therapeutic targets in a variety of diseases including cancer, cardiovascular disease, and even HIV.
Currently, there are a variety of methods used to detect PPIs, each with its own strengths and weaknesses. This is especially true in regards to sensitivity and specificity. However, the ideal assay is a cell-based, homogenous assay that maintains both its specificity and sensitivity.
Here we show that both the AlphaLISA and LANCE TR-FRET technologies allow for the development of protein: protein interaction assays in cell-based, homogenous no-wash formats suitable to HTS applications.
Related content from this organisation
- Application note: Functional GPCR studies using AlphaScreen cAMP Detection Kit
- LANCE TR-FRET
- Application note: LANCE Ultra TR-FRET –based detection and modulation of Phosphorylated STAT3 levels in human cells
- Application note: Fc-Receptor binding in ADCC assays utilising LANCE Ultra Technology – characterisation of hIGs and FcγRIIIa
- Application note: High throughput quantitation of cytokine biomarkers using LANCE Ultra TR-FRET assays