Application note: Validated quantitation and activity assay of antibody fragment molecule (Fab) for process development and quality control

Posted: 8 September 2020 | | No comments yet

This application note by ForteBio explores antibody fragment molecule (Fab) for process development and quality control.

The analytical group at Boehringer Ingelheim, Fremont, US, needed a robust assay to measure the biological activity of an antibody fragment (Fab) molecule for in-process testing, as well as stability and lot release testing in their quality control (QC) department.

Octet platform solution

The group was able to develop a working Fab activity assay on the Octet® RED system in less than a week. Relative to the overnight incubation and four-hour assay time of their ELISA protocol, the Octet assay provided an analysis time of only one hour per 96-well microplate, including sample preparation time.

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