Application note: Measure viral-induced cytopathic effects with a quantitative luminescence assay
Posted: 28 July 2021 | Molecular Devices | No comments yet
Depletion of ATP due to viral-induced CPE leads to a reduction in luminescence signal, enabling quantitation of viral-induced CPE in host cells.
Viral infection of mammalian cells often reduces cell viability and causes visible effects upon the cells such as changes in shape or size, or fusion with adjacent cells. These changes are called cytopathic effects (CPE), and they can be assessed using light microscopy or imaging systems, or measured using more quantitative means. Here we demonstrate how the Viral ToxGlo assay is used to measure viral infectivity and tissue culture infective dose (TCID50) in mammalian cells.
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