Application note: Measure viral-induced cytopathic effects with a quantitative luminescence assay

Posted: 28 July 2021 | | No comments yet

Depletion of ATP due to viral-induced CPE leads to a reduction in luminescence signal, enabling quantitation of viral-induced CPE in host cells.

Viral infection of mammalian cells often reduces cell viability and causes visible effects upon the cells such as changes in shape or size, or fusion with adjacent cells. These changes are called cytopathic effects (CPE), and they can be assessed using light microscopy or imaging systems, or measured using more quantitative means. Here we demonstrate how the Viral ToxGlo assay is used to measure viral infectivity and tissue culture infective dose (TCID50) in mammalian cells.

    To read this application note in full, please complete the form below:


    This specialist content is provided to you free-of-charge thanks to the kind support of Molecular Devices

    By clicking submit you confirm that you accept our terms and conditions and privacy policy.

    Leave a Reply

    Your email address will not be published. Required fields are marked *

    This site uses Akismet to reduce spam. Learn how your comment data is processed.