article
Under the microscope: CROs and microplate reader requirements
Contract Research Organisations rely on microplate readers for effective hit discovery. Ian Waddell, of Charles River Laboratories, explains what his needs are.
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Assays
An assay is an analytical procedure in laboratory medicine, pharmacology and molecular biology for measuring the activity of a target entity.
article
A high-throughput approach for measuring intracellular ATP levels to access compound-mediated cellular toxicity
Many drugs fail in clinical trials due to toxicity-related issues; therefore, drug-mediated cellular toxicity should be determined at an early stage in the drug discovery value chain. There are many assay systems that can be utilised to measure cellular toxicity. In this article we demonstrate that intracellular ATP concentrations are…
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Application note: Production of human induced pluripotent stem cell-derived cortical neurospheres in the DASbox® Mini Bioreactor System
There is substantial interest in the scientific community in using human induced pluripotent stem cell (hiPSC)-derived neural cells to study basic mechanisms of brain development, neuronal function, and drug-induced effects. In vitro-generated brain organoids are promising models, because they show a remarkable degree of maturation and can be kept in…
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Application note: Sensitive fluorometric quantification of DNA in microplate format
This technical note demonstrates the feasibility of using the Thermo Scientific™Fluoroskan™ or Fluoroskan FL instruments with the Invitrogen™ Quant-iT™ dsDNAhigh-sensitivity assays. Sensitive and specific quantification of double-stranded DNA is carried out in microplate format. This is part of a series of technical notes aiming to help customers choose the best…
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Application note: A novel solution to expedite antibody discovery
With breakthroughs in molecular engineering and antibody humanisation, monoclonal antibodies (mAb) are one of the fastest-growing classes of biopharmaceuticals for multiple clinical indications including cancer, cardiovascular disease, autoimmune disorders and infectious disease. Most therapeutic antibody candidates are initially generated using hybridoma technology or primary B-cell screening after antigen immunisation.
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Application note: Direct measurements of cellular metabolism for identification of mitochondrial drug targets
Drug target identification has a key role in the drug discovery value chain. A critical step in the development of pharmaceuticals is identifying the direct targets of potential drug candidates as well as distinguishing any secondary or off-target effects...
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Application note: automated cell transfection and reporter gene assays
Reporter gene assays have greatly helped our understanding of gene regulation and signal transduction in vitro and in vivo.
video
Customer testimonial PHERAstar FSX and CLARIOstar
Laura Kilpatrick, Chloe Peach and Carl White from the University of Nottingham talk about cancer research and how they use our microplate readers PHERAstar and CLARIOstar.
article
Drug Target Review – Issue 4 2018
In this issue: Omics-informed drug target discovery in combating emerging infectious diseases, measuring intracellular ATP levels to access compound-mediated cellular toxicity, and turning organoids into physiologically relevant high-content assays for drug discovery.
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Application note: PHERAstar® measures AlphaScreen assay to develop selective inhibitors for the human YEATS domains
YEATS domains are epigenetic regulators and are recognised as readers of histone post-translational modifications (HPTM) alongside bromodomains, PHD fingers, and others.
video
GPCR binding and signalling studies with the PHERAstar
This video will show how you can do GPCR binding and signalling studies with the PHERAstar.
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Application note: Cellular dopamine and intracellular calcium signaling using the next generation HTS microplate reader
This application note highlights the direct optic bottom reading performance of the BMG LABTECH microplate readers in two different cell-based fluorescence intensity assays from Invitrogen.
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Application note: Promega’s ADP-Glo™ assay
This application note discusses how the ADP-Glo™ Kinase assay is a universal, homogeneous, high-throughput screening method that measures kinase activity by quantifying the amount of ADP produced during the kinase reaction.
webinar
High throughput screening for deep mining of antibody repertoires, Dr. Stefan Moese, Novartis, Switzerland
9 November 2018 | By Beckman Coulter
In this webinar you learn about a high throughput screening approach for mining of antibody repertoires in the context of biologics drug discovery.
webinar
Harmonising drug-target binding data analytics on a single platform
6 November 2018 | By PerkinElmer
This webinar outlined the fundamentals of a design process where an experimental analytics data workflow is being integrated into a more seamlessly interactive platform...
