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Application note: Western blot & IP assay to verify antibody specificity

Posted: 1 June 2021 | | No comments yet

Learn about a practical, high-throughput method for validating the target-specificity of antibodies for the application of western blot.

Life scientists are raising awareness of the problem of irreproducible data in basic research.  Part of the crisis of irreproducibility has been blamed on antibodies, and from this standpoint, an equal push to bring awareness to antibody validation has been initiated.  Although scientists agree the onus of validation is on the user, it is also viewed that commercial antibody providers should share in this responsibility.  This application note presents a practical, high-throughput method for validating the target-specificity of antibodies for the application of western blot.












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