Apilimod small molecule shows potent inhibition of SARS-CoV-2 in cells
Researchers have found the small molecules Apilimod and Vacuolin-1 inhibited the PIKfyve kinase in cells, preventing infection from Zaire ebolavirus and SARS-CoV-2.
Researchers report that they have used small molecules to successfully inhibit infection in cells from both the Zaire ebolavirus and SARS-CoV-2, the virus causing the COVID-19 pandemic.
Conducted by researchers from Harvard Medical School and Washington University in St Louis, both US, the study showed that the Apilimod and Vacuolin-1 small molecules were effective at preventing infection.
According to the team, the small molecules were tested on flow cytometry-based assays and cell-based assays. The compounds showed potent inhibitory effects on the content release and infection by chimeric vesicular stomatitis virus (VSV), which contained the envelope proteins of Zaire ebolavirus or SARS-CoV-2. They found that Apilimod was particularly potent against SARS-CoV-2.
The researchers say these compounds inhibit the main endosomal phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase (PIKfyve). This then prevented the release of the viral contents from endosomes. Both inhibitory compounds caused the distension of Rab5 and Rab7 subcompartments into small vacuoles, say the researchers.
“These results define tools for studying the intracellular trafficking of pathogens elicited by inhibition of PIKfyve kinase and suggest the potential for targeting this kinase in developing small-molecule antivirals against SARS-CoV-2,” write the researchers in their paper.
The team add that Apilimod is safe and well-tolerated, as this has been proven in Phase I and II trials. However, this study was discontinued due to a lack of effectiveness on the autoimmune condition it was tested against. Therefore, it could prove to be a potent therapy for COVID-19.
The findings were published in Proceedings of the National Academy of Sciences in the United States of America.