Application note: High-content phenotypic profiling using the cell painting assay
The cell painting assay uses up to six fluorescent dyes to label and visualize a variety of subcellular structures at the single cell level.
High-content phenotypic profiling is increasingly popular in research areas that span from gene function studies, drug discovery and toxicology. Here, we show a high-content phenotypic profiling workflow based on the cell painting protocol and demonstrate that the proposed workflow is a user-friendly and robust approach for performing high-content phenotypic profiling.
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Analytical Techniques, Antibodies, Antimicrobials, Artificial Intelligence, Assays, Bioengineering, Biomarkers, Biopharmaceuticals, Disease Research, Genome Editing, Imaging, Immunology, Informatics, Lab Automation, Microscopy, Proteomics, Stem Cells, Targets, Toxicology